6. Surfen, a proteoglycan antagonist, reduces lysolecithin-induced demyelination with related effects on macrophage function

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Surfen, a proteoglycan binding agent, reduces inflammation but inhibits remyelination in murine models of Multiple Sclerosis

Proteoglycans are promising therapeutic targets in Multiple Sclerosis (MS), because they regulate many aspects of the immune response. This was studied using surfen, an agent that binds both heparan sulphate proteoglycans (HSPGs) and chondroitin sulphate proteoglycans (CSPGs). Initial cell culture work on bone marrow derived macrophages (BMDMs) found that surfen reduced concentrations of the ch...

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Lysolecithin-induced demyelination in primates: preliminary in vivo study with MR and magnetization transfer.

PURPOSE To study bystander demyelination in multiple sclerosis with an experimental in vivo model of toxic demyelination. METHODS Toxic demyelinating lesions were created in two monkeys by injection of lysophosphatidylcholine in the centrum semiovale. Follow-up was done clinically and with serial MR studies, including T2-weighted and gadolinium-enhanced T1-weighted images and measurement of m...

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Electrophysiological and Histological Study of Lysolecithin-Induced Local Demyelination in Adult Mice Optic Chiasm

Introduction: Demyelination is one of the main causes of neurological disability. It is the end product of numerous pathological processes, multiple sclerosis (MS) being the most common cause. More than 70% of the MS patients suffer from optic disturbances. This disease commonly affects the optic pathway, particularly the optic nerves and chiasm. Several attempts have been made to produce a ...

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Surfen, a small molecule antagonist of heparan sulfate.

In a search for small molecule antagonists of heparan sulfate, we examined the activity of bis-2-methyl-4-amino-quinolyl-6-carbamide, also known as surfen. Fluorescence-based titrations indicated that surfen bound to glycosaminoglycans, and the extent of binding increased according to charge density in the order heparin > dermatan sulfate > heparan sulfate > chondroitin sulfate. All charged gro...

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Comparision of the effects of Leishmania Soluble Antigen (LSA) and Lipopolysaccharide (LPS) on C57BL/6 Mice Macrophage Function

Background: Macrophages activation is the important anti-leishmania immune response. Different signals could affect macrophages development and functional activation. Objectives: In the present study, we compared the effect of Leishmania Soluble Antigen (LSA)and Lipopolysaccharide (LPS) on peritoneal macrophage responses. Appropriate activation of macrophages depends on thesignals they receive ...

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ژورنال

عنوان ژورنال: Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques

سال: 2015

ISSN: 0317-1671,2057-0155

DOI: 10.1017/cjn.2015.254